Overview | soybean saponins (soy saponins), also known as soybean saponins, extracted from the seeds of soybean and other legumes, it is a class of compounds formed by the condensation of oligosaccharides and oleanolic triterpenes. The content of soybean saponin in the seeds of bean plants is generally between 0.62 and 6.16%. Studies at home and abroad have proved that soybean saponins have many physiological functions, such as anti-lipid oxidation, anti-free radicals, enhancing immune regulation, anti-tumor, anti-virus and so on. At present, it has been used in food, medicine, cosmetics and other fields, and has broad market prospects. |
preparation | soybean saponin BE methyl ester preparation as follows: sample soybean saponin raw material concentration of 6.5mg/ml, the content of soybean saponin in dry matter was 1.5%, the sample volume was 1000ml, and the sample and elution temperature were 80. The crude soybean oligosaccharide product was obtained by washing with 460ml water first, combining the sample effluent and water wash to dry; The adsorption column was washed with 1400ml of 35% ethanol; Finally, the column was eluted with 1200ml of 60% ethanol at a flow rate of 8.0ml/min, the soybean saponin eluate was collected and the elution volume was 1150ml. Vacuum rotary evaporation, soybean saponin crude 13.13g, Brown yellow, content of 45.5%. The yield was 91.9%. Put the above crude soybean saponin into a 250ml round bottom flask, add 50ml of 40% ethanol, heat reflux, temperature is 85 deg C, then poured into the centrifugal tube, 30 deg C cooling 24H, the mother liquor and crystallization were separated by centrifugation, washed with water, centrifuged again to obtain the crystalline product, and dried to obtain 3.32g of soybean saponin BE methyl ester. |